Volume 1041, Issue 1 p. 292-295

Signaling Pathways of the LGR7 and LGR8 Receptors Determined by Reporter Genes

MICHELLE L. HALLS

MICHELLE L. HALLS

Department of Pharmacology, Monash University, Clayton, Victoria 3800, Australia

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ROSS A. BATHGATE

ROSS A. BATHGATE

Howard Florey Institute, Melbourne University, Parkville, Victoria 3010, Australia

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PETER J. ROCHE

PETER J. ROCHE

RMIT University, Bundoora, Victoria 3083, Australia

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ROGER J. SUMMERS

Corresponding Author

ROGER J. SUMMERS

Department of Pharmacology, Monash University, Clayton, Victoria 3800, Australia

Address for correspondence: Prof. Roger J. Summers, Department of Pharmacology, PO Box 13E, Monash University, Wellington Rd, Clayton, Victoria 3800, Australia. Voice: +61 3 9905 1440; fax: +61 3 9905 8192. [email protected]Search for more papers by this author
First published: 09 January 2006
Citations: 9

Abstract

Abstract: Although much is known about the pleiotropic effects mediated by relaxin, the exact signaling pathways involved remain relatively elusive. This study examines LGR7 and LGR8 signaling using reporter gene technology. The greatest response was observed at the CRE reporter (indicates activation of cAMP-PKA and p38/JNK pathways), although INSL3 treatment of LGR8 produced a lower response than H2 relaxin treatment of LGR7. AP1 (which indicates activation of JNK pathways) was stimulated to a lesser degree. Three other reporters produced no response. The reporter gene studies suggest that ligand stimulation of LGR7 and LGR8 involves cAMP-PKA and p38/JNK signaling.